In vivo pharmacological evidence and biodistribution. These results only indicate the perceptions of the website users. Sialylation enables these cells a long lifetime in circulation 6, 16a, 17. The nano particle suspension was placed onto a 400 mesh copper grid coated with carbon. Sialic acid synthesis and signaling biofiles volume 5. This gene encodes an enzyme which removes 9oacetylation modifications from sialic acids. Development of sialic acid coated nanoparticles for targeting cancer and efficient evasion of the immune system. Younghwa kim 1, kyung hyun min 1, zhantong wang 1, jihoon kim 1, orit jacobson 1, peng huang 2, guizhi zhu 1, yijing liu 1, bryant yung 1, gang niu 1, xiaoyuan chen 1.
Structural differences between neu5ac and neu5gc sialic acids scheme 1. Quantum dot nanometal surface energy transfer based. Sialic acidcovered nanoparticles are expected to facilitate inhibition of immune cell activation, which allows res escape by rendering the nanoparticles prolonged circulation in the blood stream and reduced uptake by the immune system. Request pdf inhibition of influenza virus infection by multivalent sialicacid functionalized gold nanoparticles an efficient synthesis of. Sialic acid is an electro negatively charged monosaccharide that targets the selectins present in the plasma membrane of cancer cells. Colorimetric viral detection based on sialic acid stabilized. Structure of influenza viruses, connected with influenza life. Sialic acids are a family of ninecarbon acidic monosaccharides that occur naturally at the end of sugar chains attached to the surfaces of cells and soluble proteins. When multivalently displayed on nanoparticles, s1 or s1a bound to human erythrocytes and to human mucin in a strictly siadependent fashion. Analysis of sialic acids using highperformance anion. Sialic acid and glycopeptides conjugated plga nanoparticles for central nervous system targeting.
The following are the results of ongoing survey on for sialic acid. Over 50 forms of sialic acid have been identified to date, due to the number of locations available for structural modification as well as core variation. Analysis of the changes in expression levels of sialic acid on influenzavirusinfected cells using lectintagged polymeric nanoparticles article pdf available in frontiers in microbiology 7. Neuraminidase has to cleave sialic acid from the surface of host cells enzymatically, before releasing of new virions 40. Sialic acids sas are typically found as terminal monosaccharides attached to cell surface glycoconjugates, which play crucial roles in various biological processes, and aberrant sialylation is closely associated with many diseases, particularly cancers. In vitro studies have shown a transient association of 40 nm polystyrene nanoparticles ps nps with these cells, which is of interest for. Therapeutic applications of selenium nanoparticles. Na is an enzyme with hydrolytic activity, cleaving the glycosidic bond between the sialic acid nacetylneuraminic acid and dgalactosamin or dgalactose, which represents ha receptor on host cell surface 54, 55. Formerly suggested as sialidasesensitive sources of anionic groups at the cell surface of fungal species grown in chemically defined media e. Role of nanoparticle size and sialic acids in the distinct timeevolution profiles of nanoparticle uptake in hematopoietic progenitor cells and monocytes december 2019 journal of nanobiotechnology. Detection of influenza virus by agglutination using. An additional factor that contributes to sialic acid complexity in vivo is the migration of an oacetyl ester moiety between c7 and c9 under certain physiological conditions.
Certain embodiments are directed to systems and methods using surface enhanced raman scattering sers detection. In this study, we fabricated lectintagged fluorescent polymeric nanoparticles approximately 35 nm in diameter using biocompatible polymers conjugated with lectins for the purpose of detecting sialic acid on a living cell surface, which is one of the most important biomarkers for cancer diagnosis. Sialic acid definition of sialic acid by medical dictionary. Plga nanoparticles surface decorated with the sialic acid, nacetylneuraminic acid. Sialic acids are ubiquitous terminal residues of glycoconjugates and occur in a wide variety as a result of modifications of the core nacetyl neuraminic acid molecule and of differences in.
People with sialic acid storage disease have signs and symptoms that may vary widely in severity. Diagnosis of breast cancer by analysis of sialic acid concentrations in human saliva by surfaceenhanced raman spectroscopy of silver nanoparticles article pdf available in nano research june. We here report a novel strategy for specific fluorescence labeling of such motifs. Sialic acid storage disease genetics home reference nih. Pdf colorimetric determination of sialic acid based on boronic. Protection against neurodegeneration in the hippocampus using sialic acid and 5htmodulineconjugated lipopolymer nanoparticles jen tsung yang, yungchih kuo, iyin chen, rajendiran rajesh, yungi lou, and jyhping hsu.
As mentioned earlier, these represent binding sites for pathogens such as the influenza virus. Pdf plga nanoparticles surface decorated with the sialic. Sialic acid was found to promote the cancer targeting, cytotoxicity and cell penetrating abilities of senps in hela cells. The functions of sialic acid can be briefly explained as, mediation of binding of some substances to cells with its presence on cell surfaces. Therefore, we opted to design a welldefined, selfassembling nano particle for arrayed presentation of s1 and s1 subdomains. Role of nanoparticle size and sialic acids in the distinct time. Application of sialic acidpolysialic acid in the drug delivery systems. Sialic acidimprinted fluorescent coreshell particles for.
Pdf development of sialic acidcoated nanoparticles for. May, 2019 moreover, when investigating the timedependent effect of cleavage of sialic acid residues on ps np localization by means of confocal microscopy figs. Gold nanoparticles decorated with sialic acid terminated bi. A dmblabeled sialic acid reference panel profiled on an lsr1 hplc column. Apr 15, 20 sialic acid reduced and stabilized gold nanoparticles d 20. Polysialic acidbased nanoparticles for drug delivery. Pdf role of nanoparticle size and sialic acids in the. Serum total sialic acid, lipid peroxidation, and glutathione.
As sas are overexpressed in tumorassociated glycoproteins, the recognition and specific binding of sa are crucial for monitoring, analyzing. Request pdf inhibition of influenza virus infection by multivalent sialicacidfunctionalized gold nanoparticles an efficient synthesis of. Polysialic acid psa is a homopolymer of sialic acid in. Quantum dot nanometal surface energy transfer based biosensing of sialic acid compositions and linkages in biological samples raghavendra kikkeri. Linearity, lod, loq, and precision n9 for dmb derivatised sialic acid determination figure 3. Pdf analysis of the changes in expression levels of. They also provide the negative charge and hydrophilicity that contribute to the rheological. Sialic acid is a generic term for a family of derivatives of neuraminic acid, an acidic sugar with a ninecarbon backbone. Jan 04, 2017 in this work, we design and synthesize a fluorescent conjugated polymer modified with a phenylboronic acid group, which can covalently bind with cisdiolcontaining compounds, such as sialic acid sa, by forming a cyclic ester.
However, the obtained conjugated polymer nanoparticles failed to discriminate between cancer cells, with or without sa overexpressed surfaces. This paper describes a novel approach for the quantitation of nonderivatized sialic acid in glycoproteins, separated by hydrophilic interaction chromatography, and detection by nano quantity analyte. Nanoparticles conjugated with the receptormimic peptide may be a useful alternative to red blood cells in the global surveillance and clinical diagnosis of influenza. Sialic acidcovered nanoparticles are expected to facilitate inhibition of immune cell activation, which. More specifically, the present invention describes a system and method of use for sers detection of sialic nacetylneuraminic acid using citratereduced silver nanoparticles. Studies on the stability of simple derivatives of sialic acid. Detection of influenza virus by agglutination using nanoparticles conjugated with a sialic acid mimic peptide. This value was doubled in the np modified using sialic acid sag7np, suggesting the presence of a soft coating that provide several points of contact of the np with the mica support. Workflow for sialic acid analysis using ludgers dmb kit table 1. Initially demonstrated by hemagglutination assay with human erythrocytes and intact virus, merscov siabinding activity was assigned to s subdomain s1a. Sialic acids are prominently expressed along the epithelial border lining the airways and are also major components of the secreted mucins in the airways. The gold nanoparticles showed targetspecific aggregation with viral particles via hemagglutininsialic acid binding.
Role of nanoparticle size and sialic acids in the distinct timeevolution profiles of nanoparticle uptake in hematopoietic progenitor cells and monocytes. Protection against neurodegeneration in the hippocampus. Review structure, function and metabolism of sialic acids. Herein, we compare the effects of sialic acid as well as different charged pegylated nanoparticles in phagocytosis and immune activation. In addition, a few orienting studies were performed with a specimen of nacctylneuraminic acid secured from human serum proteins. Please base your medical decisions only on the advice of a doctor or a registered medical professional.
Serum sialic acid has been reported as a marker of the acute phase response. Inhibition of influenza virus infection by multivalent sialicacid. This is based on sialic acidimprinted coreshell nanoparticles equipped with nitrobenzoxadiazole nbd fluorescent reporter groups allowing. Sialic acidtargeted biointerface materials and bioapplications. The role and potential of sialic acid in human nutrition. A novel approach for quantitation of nonderivatized sialic. The expression of cell surface glycans terminating with sialic acid sa residues has been found to correlate with various disease states there among cancer. Nacetylneuraminic acid neu5ac or nana is the predominant sialic acid found in human cells, and many mammalian cells. Polymers, nanoparticles, glycans, sialic acid, influenza. Sialic acid nacetylneuraminic acid, neu5ac is recognized as a self marker by major serum protein complement factor h and shows reduced interaction with the innate immune system via sialic acidbinding immunoglobulinlike lectin siglec, which is known as one of the significant regulators of phagocytic evasion. The results suggest that modification of nanoparticles with sialic acid results in decreased clearance by the. Request pdf sialic acid modified nanoparticles for.
Such lowaffinity interactions may be augmented through multivalencydriven, highavidity binding 34 36. Bioassay systems sialic acid assay uses a single working reagent that combines nana aldolase, pyruvate oxidase and hydrogen peroxide determination in one step. Wo2017015568a1 lablefree detection of sialic acid using. Pdf the authors describe a rapid, sensitive and selective colorimetric assay for sialic acid sa based on the use of gold nanoparticles. Eselectintargeted sialic acidpegdexamethasone micelles. It is also the name for the most common member of this group, nacetylneuraminic acid neu5ac or nana. Sialic acid is an acetylated derivative of neuraminic acid and it is attached to nonreducing residues of carbohydrate chains of glycoproteins and glycolipids. This disorder is generally classified into one of three forms. Development of sialic acidcoated nanoparticles for. Identification of sialic acidbinding function for the middle. These findings could be considered as the evidence on the molecules of sa on the np surface. Sialic acid storage disease is an inherited disorder that primarily affects the nervous system.
Thus, the sialic acid chains must be attached to a linker molecule to generate an active bioconjugate that is able to inhibit the release of net. Role of nanoparticle size and sialic acids in the distinct. Synthesis of silver nanoparticles and the development. Preparation of epigallocatechin gallateloaded nanoparticles. Schauer structure, function and metabolism of sialic acids figure 2. Lectintagged fluorescent polymeric nanoparticles for. Therefore, dexamethasonedxmloaded eselectintargeting sialic acid polyethylene glycoldexamethasone sapegdxmdxm conjugate micelles are designed for ameliorating aki. Network accelerates innovation by allowing you to discover and connect to gamechanging technologies and technology professionals on the worlds most comprehensive technology network. The sialic acid containing sugar chains play a role in functions, such as intercellular signal transduction and receptors receptorlike functions for binding of some substances to cells.
Sialic acid sa is main component of sialyl lewis x antigen on the mammalian cell surface, which participates in eselectin binding. Application of sialic acidpolysialic acid in the drug. Identification of sialic acidbinding function for the. We have applied colloid silver nanoparticles as colorimetric sensor of sialic acid and melamine, the presence of sialic acid and melamine induces the. This minimizes reuptake of the associated therapeutic drug and thereby allows for extended circulatory stability while maintaining sustained therapeutic release. Whilst all hemagglutinins bind sialic acids, human influenza targets terminal. Pegylation nanoparticles can effectively reduce the macrophage uptake in vitro, prolong the circulation halflife in vivo and decrease the accumulation of. However, the obtained conjugated polymer nanoparticles failed to discriminate between cancer cells, with or without. Other forms, such as nglycolylneuraminic acid, may also occur in cells. The morphology of the prepared nano particles was examined by tem at different phs. Therefore, it is important to know the sialic acid content of a glycoprotein when assaying its function or its efficacy as a pharmaceutical therapeutic. Development of sialic acidcoated nanoparticles for targeting. Preparation of sialic acidimprinted fluorescent conjugated.
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